
Cell Culture Media, Supplements, and Reagents
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Gibco™ RPMI 1640 Medium
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer.


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Shipping Condition | Room Temperature |
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Cell Line | HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas |
Form | Liquid |
Without Additives | No HEPES,No Sodium Pyruvate |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
Concentration | 1 X |
Sterility | Sterile-filtered |
Cell Type | Leukemic Cells |
Sterilization Method | Sterile-filtered |
With Additives | Glutamine,Phenol Red |
Product Type | RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium) |
Classification | Animal Origin-free |
Shelf Life | 12 Months From Date of Manufacture |
Product Line | Gibco™ |
Serum Level | Standard Serum Supplementation |
Gibco™ OptiPRO™ SFM
OptiPRO™ SFM is a serum-free, animal origin-free culture medium designed for growth of several kidney-derived cell lines including MDBK, MDCK, PK-15 and VERO for virus or recombinant protein production.

Shipping Condition | Room Temperature |
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Cell Line | HeLa, MDCK, BHK-21, PK-15, COS-7, MDBK, VERO |
Content And Storage | Storage conditions: 2 – 8°C. Protect from light. Shipping conditions: Ambient Shelf life: 24 months from date of manufacture |
Form | Liquid |
Without Additives | No Glutamine |
Endotoxin Level | Low |
Concentration | 1 X |
Format | Bottle(s) |
Included Antibiotics | No antibiotics |
Product Type | Serum Free Medium |
Classification | Animal Origin-free,Low Protein,Serum-free |
Culture Type | Adherent Cell Culture |
Product Line | Gibco™ |
Serum Level | Serum-free |
Gibco™ Hybridoma-SFM
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Specifically developed to support the growth of hybridomas for antibody production


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Room Temperature |
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Content And Storage | Storage conditions: 2-8°C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
With Additives | Glutamine,Phenol Red |
Product Type | Serum Free Medium (SFM) |
Form | Liquid |
Classification | Low Protein,Serum-free |
Product Line | Gibco™ |
Serum Level | Serum-free |
Cell Type | Hybridomas |
Gibco™ Sf-900™ II SFM
Optimized for large-scale production of recombinant proteins expressed using the baculovirus expression vector system (BEVS)


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Room Temperature |
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Cell Line | Sf21, Sf9 |
Content And Storage | Storage conditions: 2°C to 8°C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
With Additives | Glutamine |
Product Type | Insect Cell Serum Free Medium (SFM) |
Form | Liquid |
Classification | Protein-free,Serum-free |
Species | S. frugiperda, Spodoptera frugiperda |
Product Line | Gibco™, Sf-900™, SynerGy™ |
Serum Level | Serum-free |
Cell Type | Insect Cell |
Gibco™ Neurobasal™-A Medium
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Neurobasal™-A Medium


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Gibco™ MEM Non-Essential Amino Acids Solution (100X)
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Gibco™ MEM Non-Essential Amino Acids are used as a supplement for cell culture medium, to increase cell growth and viability.


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Content And Storage | Store at 2–8°C. Protect from light. |
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Product Type | Amino Acid Supplement |
Form | Liquid |
pH | 0.9 |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
Shelf Life | 18 Months |
Concentration | 100X |
Sterility | Sterile-filtered |
Cell Type | Mammalian Cells |
Gibco™ StemPro™-34 SFM (1X)
A serum-free medium specifically formulated to support the development of human hematopoietic cells in culture.


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Gibco™ HT Supplement (100X)
HT-supplemented medium is suitable for post-selection rescue to overcome the effects of residual intracellular aminopterin.


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Gibco™ Trypsin-EDTA (0.5%), no phenol red
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas


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Gibco™ Advanced DMEM/F-12
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Advanced DMEM/F-12 (Dulbecco's Modified Eagle Medium/Ham's F-12) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.


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Shipping Condition | Room Temperature |
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Cell Line | MRC-5, SP2, Vero, WI-38 and Jurkat |
Content And Storage | Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
Form | Liquid |
Without Additives | No Glutamine,No HEPES |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
Concentration | 1 X |
Sterility | Sterile-filtered |
Sterilization Method | Sterile-filtered |
With Additives | High Glucose,Phenol Red,Sodium Pyruvate,Non Essential Amino Acids (NEAA) |
Product Type | DMEM (Dulbecco's Modified Eagle Medium)/F-12 |
Classification | Animal Origin |
Shelf Life | 12 Months From Date of Manufacture |
Product Line | Gibco™ |
Serum Level | Reduced Serum |
Gibco™ FreeStyle™ 293 Expression Medium
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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An animal origin-free, serum-free, protein-free formulation, allowing for easier purification


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Shipping Condition | Room Temperature |
---|---|
Content And Storage | Storage conditions: 2°C to 8°C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
Product Type | Expression Medium |
Form | Liquid |
Classification | Animal Origin-free,Chemically-defined,Protein-free,Serum-free |
Product Line | Freestyle™ |
Serum Level | Serum-free |
Gibco™ IMDM
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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IMDM (Iscove's Modified Dulbecco's Medium) is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Room Temperature |
---|---|
Cell Line | Jurkat, COS-7, and macrophage cells |
Content And Storage | Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
Form | Liquid |
Without Additives | No α-Thioglycerol,No 2-Mercaptoethanol |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
Concentration | 1 X |
Sterility | Sterile-filtered |
Sterilization Method | Sterile-filtered |
With Additives | High Glucose,Glutamine,HEPES,Phenol Red,Sodium Pyruvate |
Product Type | IMDM (Iscove's Modified Dulbecco's Medium) |
Classification | Animal Origin-free |
Shelf Life | 12 Months From Date of Manufacture |
Product Line | Gibco™ |
Gibco™ Opti-MEM™ Reduced Serum Medium, powder
Opti-MEM™ Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change to growth rate or morphology.


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Shipping Condition | Room Temperature |
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Cell Line | Sp2, AE-1, CHO, BHK-21, and HEK |
Content And Storage | Storage conditions: 2-8° C Shipping conditions: Ambient Shelf life: 30 months from date of manufacture |
With Additives | Glutamine,Phenol Red |
Product Type | Opti-MEM I |
Form | Powder |
Classification | Animal Origin |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
Shelf Life | 30 Months From Date of Manufacture |
Sterility | Sterile-filtered |
Product Line | Gibco™, Opti-MEM™ |
Cell Type | Primary Fibroblasts |
Gibco™ Glasgow's MEM, powder
Originally developed by Ian McPherson and Michael Stoker as modification of Eagle's medium

Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Gibco™ Ham's F-12 Nutrient Mix, powder
Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells.

Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Room Temperature |
---|---|
Content And Storage | Storage conditions: 2-8° C Shipping conditions: Ambient Shelf life: 36 months from date of manufacture |
With Additives | Glutamine,Phenol Red,Sodium Pyruvate |
Product Type | Ham's F-12 Nutrient Mixture |
Form | Powder |
Without Additives | No HEPES,No Sodium Bicarbonate |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
Product Line | Gibco™ |
Cell Type | Primary Rat Astrocytes |